Volocity® Acquisition stands as a pinnacle of innovation, meticulously crafted to facilitate lightning-fast, three-dimensional image capture. This sophisticated software solution offers an unparalleled degree of flexibility, seamlessly interfacing with an extensive array of cameras, microscopes, and complementary hardware. This empowers you to curate a bespoke setup tailored precisely to your unique application, without any concessions.

The software's interface is designed with utmost clarity and user-friendliness, enabling the swift construction of capture protocols. From fundamental two-dimensional image acquisition to the complexities of multi-channel four-dimensional experiments, Volocity® Acquisition's intuitive Acquisition dialogue guides you through the process effortlessly. It's a tool designed to augment your scientific pursuits by providing the control and precision necessary for extracting valuable insights from your imaging endeavors.

Flexible protocol design for all your imaging experiments:

Tailor your experiment to the biology: Volocity Acquisition is configurable with different hardware, and once set up in preferences you can construct complex and varied acquisition and analysis protocols without the need for complex macro writing skills.

Image acquisition made easy:

View your sample in the Video Preview before you start to capture images. You have the option to apply a colour look-up table and Auto Contrast to both the Video Preview and to the images as they are acquired. Auto Contrast allows you to set a shorter camera exposure without reducing the apparent brightness of the image – ideal for fast focusing or very rapid image acquisition. Customize the hardware interface controls on the Video Preview palette to hide items you don’t use, so that navigation is easier. Once you have set up your acquisition hardware for a particular task, you can save the settings in the Light Path Manager. Properties that can be saved include filter position, objective turret, camera exposure and binning for use with a particular fluorochrome. This means that when you want to use the same settings again, you can simply click on the appropriate light manager button and they will be restored, saving you time and providing consistency.

Multi-parameter protocols:

Protocols that  involve scanning  multiple points on a well plate or slide are easy to configure by first creating a Well Overlay of the selected or random points from which you wish to acquire images. Well overlays can be saved for reuse. By selecting the XY Stage in the Acquisition Setup dialog, images will be captured at the points set in the well overlay. The acquisition protocol can include image capture at multiple wavelengths, multiple time points and in 3D at each point you have selected, for complete flexibility. Supports XYZ stitching for high resolution imaging in large fields of view.

FRAP and Ratio Acquisition:

Volocity Acquisition can be used to acquire and analyze FRAP and ratiometric  imaging experiments,  by adding  the optional  FRAP Plugin and Ratio Plugin to your system. Volocity Quantitation must be included in the Volocity configuration in order to perform analysis.